Diagnostic methodology of clinical biochemistry and molecular biology

Module Diagnostic molecular methodology

The student must have developed and acquired theoretical, scientific and professional knowledge for the understanding of the analysis methodologies for the analysis and identification of nucleic acids and their use in diagnostics. The student must acquire specific areas of expertise in the knowledge of the molecular methodologies used in the diagnosis of human pathologies, with particular reference to tumors. Finally, the student must have acquired skills in the critical processing of the results obtained through basic (PCR) and advanced (microarray and sequencing) molecular investigations as well as understanding similar data present in the literature.

The teaching activity will be frontal and integrated with some seminars. Further in-depth material will also be distributed (scientific articles on specific topics).

Knowledge of basic scientific subjects (biology and molecular biology)

MANDATORY attendance as established by the course regulations.

The course aims to deepen the basic and advanced molecular diagnostic methods, namely the principle and functioning of polymerase chain reaction (PCR), nested PCR, real-time PCR, droplet digital PCR, microarray platforms and old and new generation sequencing platforms. These topics will be discussed from a technical and applicative point of view.

Medicina di Laboratorio - Logica & Patologia Clinica. Piccin. Autori Antonozzi - Gulletta